Q. Methionine amino peptidase will remove the N terminal formyl methionine if the N+1 amino acid is_________

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Q. The f-Met tRNA binds to ribosomal _______ site.

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Q. The 50S ribosomal subunit is folded in ______ major domains.

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Q. State whether the enzyme PDF is a novel drug target for antibiotics.

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Q. The Shine and Dalgarno is located in ____________

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Q. The catalytic activity of the ribosome is located in the ______

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Q. Puromycin is a ribosomal antibiotic. It binds to ________

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Q. In an experiment you add rifampicin and paramomycin to the ribosome and mRNA mixture. What will inhibit theribosomal translation efficiency and how?

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Q. Which antibiotic inhibits peptidyl transfer by blocking the accommodation of aa-tRNA into the peptidyl transferase (A/A) site?

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Q. Which of the antibiotic can halt translocation of the ribosome bound to the mRNA?

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Q. The ribosome slides over the mRNA translating it.

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Q. In an experiment you add streptomycin at low concentration first and then gradually increase the concentration till it completely inhibits peptide formation. What will be its effect at low concentration?

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Q. What will be DNA sequence of Shine and Dalgarno coding region?

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Q. In an experiment you grow some cells in a media containing heavy isotopes of C and N. Then you transfer then to a lighter media. If you centrifuge the ribosomes so obtained after an hour how many bands will you expect?

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Q. In an experiment you stall the ribosome with low Mg²⁺ concentration. Which of the following will then cause the release of the Peptide from the ribosome?

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Q. What discovery was stated by Robert Traut and Munro experiment?

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Q. What was the unnatural experimental condition which proved to be a criticism in Robert Traut and Munro experiment?

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Q. Which of the following was not a protein degrading agent used by Noller in his experiment?

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Q. You gradually treat ribosome with SDS followed by Proteinase K and the finally extract it with phenol. Which of the options match the result you expect?

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Q. How many proteins were still intact after the treatment of ribosome with SDS and proteinase K?

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