Q. You are checking the sequence of 3 genes using generalized transduction mapping. The recipient is Leu-, Aziˢ, Thr- while the donor is Leu+, Aziᴿ, Thr +. If you select the Leu+ as marker, Aziᴿ would be 50% while Thr+ would be 2%. If you select Thr+ as marker Leu+ would be 3% and Aziᴿ would be 0%. What is the sequence?

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Q. In a transduction experiment where the recipient is Met-, Ala-, Gal- you observe a recombinant phenotype Met+, Gal+ in some strains and Gal+ Ala+ in some strains. But in none of the cases can you find Met+ Ala+. What is the sequence of the gene?

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Q. What is the capacity of viral gene storage termed as?

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Q. Which of the following viruses will be useful for transduction?

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Q. If co-incorporation probabilities are given as p,o 0.1; o,r 0.02 ; p,r 0.15. What is the gene order?

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Q. Probability of incorporation of a gene P is 0.2 and the probability of incorporation of another gene is 0.1. The probability of both being incorporated is seen experimentally to be equal to 0.1. What is our inference?

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Q. If the probability of incorporation of a gene A from a bacterial cell that has lysed in the medium is a. And the probability of incorporation of another gene B from the same bacteria that has lysed is b. What is the probability of one recipient receiving both?

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Q. What is the minimum length of DNA fragment in exogenote required for incorporation of the gene in the endogenote?

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Q. If a bacterium is heterozygous for a gene then will both the genes have an equal probability of incorporation?

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Q. Competence can be generated in some strains of non-competent bacteria by __________

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Q. Which of the following species has natural competence?

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Q. If the genes order in bacteria is His gal met. Which of the following genes have the least probability of getting incorporated alone?

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Q. State whether it is true or false- Broad scale mapping can be done using transformation?

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Q. In an experiment you have a U-Tube with two strains of bacteria in two arms. In one arm the bacteria are Gal+ and His-, in other the strain is Gal- and His+. If the two arms are joined by a membrane that doesn’t allow bacterial cells to pass to opposite site what will you observe when you plate each arm bacterial strain on minimal media?

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Q. You study the gene incorporation in recipient using HFR and get the following sequences-
P-W-A-K, W-L-I-K, A-K-I-L, W-P-R-T-W-L. What is the order of the gene?

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Q. For complete HFR to enter the recipient, how much time should the two bacterial cells be in contact?

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Q. In an experiment of determining the order of gene in bacteria we use HFR which is gal+, Lac+, Ara+ and His+. If the recipient is negative for all these genes, and we selectively choose Gal + and get the pattern-

100 % recipients are Gal +
30% are His+
55% are Lac +
And only 5% are Ara+

What would be our inference?

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Q. If genes enter the different F- cells from different HFRs in the order as shown-

Azi 8 88

Ton 10 90

Lac 17 3

Gal 24 11

What is the order of the gene where columns are for different HFR and values denote time?

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Q. In and interrupted mating experiment, it is seem that the after 10 mins Aziᴿ gene in recipient, after 20 minuites there is appearance of tonᴿ and after 15 mins the recipient is lac⁺ , but the recipient is almost never his⁺. Where in the genome is the ori of transfer?

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Q. All the genes in the HFR that is transferred to the recipient cell before breakage of the conjugation channel is incorporated in the recipient genome. State whether it is true or false.

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