Q. Which of these is correct according to transduction?

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Q. The Phage is incorporated in the bacterial genome by _____________________

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Q. Which of the following is not true for specialized transduction?

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Q. You are checking the sequence of 3 genes using generalized transduction mapping. The recipient is Leu-, Aziˢ, Thr- while the donor is Leu+, Aziᴿ, Thr +. If you select the Leu+ as marker, Aziᴿ would be 50% while Thr+ would be 2%. If you select Thr+ as marker Leu+ would be 3% and Aziᴿ would be 0%. What is the sequence?

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Q. In a transduction experiment where the recipient is Met-, Ala-, Gal- you observe a recombinant phenotype Met+, Gal+ in some strains and Gal+ Ala+ in some strains. But in none of the cases can you find Met+ Ala+. What is the sequence of the gene?

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Q. What is the capacity of viral gene storage termed as?

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Q. Which of the following viruses will be useful for transduction?

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Q. If co-incorporation probabilities are given as p,o 0.1; o,r 0.02 ; p,r 0.15. What is the gene order?

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Q. Probability of incorporation of a gene P is 0.2 and the probability of incorporation of another gene is 0.1. The probability of both being incorporated is seen experimentally to be equal to 0.1. What is our inference?

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Q. If the probability of incorporation of a gene A from a bacterial cell that has lysed in the medium is a. And the probability of incorporation of another gene B from the same bacteria that has lysed is b. What is the probability of one recipient receiving both?

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Q. What is the minimum length of DNA fragment in exogenote required for incorporation of the gene in the endogenote?

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Q. If a bacterium is heterozygous for a gene then will both the genes have an equal probability of incorporation?

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Q. Competence can be generated in some strains of non-competent bacteria by __________

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Q. Which of the following species has natural competence?

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Q. If the genes order in bacteria is His gal met. Which of the following genes have the least probability of getting incorporated alone?

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Q. State whether it is true or false- Broad scale mapping can be done using transformation?

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Q. In an experiment you have a U-Tube with two strains of bacteria in two arms. In one arm the bacteria are Gal+ and His-, in other the strain is Gal- and His+. If the two arms are joined by a membrane that doesn’t allow bacterial cells to pass to opposite site what will you observe when you plate each arm bacterial strain on minimal media?

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Q. You study the gene incorporation in recipient using HFR and get the following sequences-
P-W-A-K, W-L-I-K, A-K-I-L, W-P-R-T-W-L. What is the order of the gene?

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Q. For complete HFR to enter the recipient, how much time should the two bacterial cells be in contact?

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Q. In an experiment of determining the order of gene in bacteria we use HFR which is gal+, Lac+, Ara+ and His+. If the recipient is negative for all these genes, and we selectively choose Gal + and get the pattern-

100 % recipients are Gal +
30% are His+
55% are Lac +
And only 5% are Ara+

What would be our inference?

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