Question

Which of the following is not included as a step in cloning in bacteriophage PI?

a.

The growth of vector is done as a plasmid by using pBR322 origin of replication

b.

Cleaving with BamH1 is done and then products cleaved with Sau3A are ligated

c.

Incubation with mutant lysogen is done which doesn’t cleaves the recombinants at pac sites

d.

Incubation is done with mutant lysogen which doesn’t contains head and tail proteins

Posted under Genetic Engineering

Answer: (c).Incubation with mutant lysogen is done which doesn’t cleaves the recombinants at pac sites

Interact with the Community - Share Your Thoughts

Uncertain About the Answer? Seek Clarification Here.

Understand the Explanation? Include it Here.

Q. Which of the following is not included as a step in cloning in bacteriophage PI?

Similar Questions

Explore Relevant Multiple Choice Questions (MCQs)

Q. What happens after packaging is carried out in the phage head?

Q. Circularization must take place in the bacterial cells before the replication starts.

Q. How many origins of replication are there once the cyclization is carried out by loxP sites?

Q. Choose the incorrect statement for PAC vectors.

Q. Choose the correct statement in respect to sacB gene?

Q. Selection of molecules by lack of inserts on the basis of the sacB gene is known as:

Q. F (fertility) factor is used in many bacterial systems for different purposes. Choose the incorrect statement with respect to the F factor.

Q. F factor integration into the host chromosome is called as __________

Q. Excision of the F factor can also be done from the host chromosome. Choose the incorrect statement in respect to it.

Q. The low copy number of F plasmid is essential for the stability of these plasmids.

Q. Choose the correct statement for BAC vector system.

Q. The bacteriophage M13 vectors belong to a group of phages called as __________

Q. Choose the correct statement for the infection process of M13.

Q. Once the bacteriophage enters the bacterial cells, some changes are carried out in it. Which of the following is not included?

Q. Replication can also be carried out by rolling circle replication. Choose the correct statement for this type of replication?

Q. Gene V blocks the production of which gene?

Q. What is the size of the DNA molecule which can be packed by the viral protein coat?

Q. The uniform layer of cells is known as lawn and the holes peppered from infected cells are termed as __________

Q. M13 doesn’t actually lyse the cells.

Q. The plaques formed by M13 infection are called as __________

Recommended Subjects

Are you eager to expand your knowledge beyond Genetic Engineering? We've handpicked a range of related categories that you might find intriguing.

Click on the categories below to discover a wealth of MCQs and enrich your understanding of various subjects. Happy exploring!