Question

Which of the following is not included as a step in cloning in bacteriophage PI?

a.

The growth of vector is done as a plasmid by using pBR322 origin of replication

b.

Cleaving with BamH1 is done and then products cleaved with Sau3A are ligated

c.

Incubation with mutant lysogen is done which doesn’t cleaves the recombinants at pac sites

d.

Incubation is done with mutant lysogen which doesn’t contains head and tail proteins

Answer: (c).Incubation with mutant lysogen is done which doesn’t cleaves the recombinants at pac sites

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Q. Which of the following is not included as a step in cloning in bacteriophage PI?

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