Q. Circularization must take place in the bacterial cells before the replication starts.

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Q. How many origins of replication are there once the cyclization is carried out by loxP sites?

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Q. Choose the incorrect statement for PAC vectors.

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Q. Choose the correct statement in respect to sacB gene?

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Q. Selection of molecules by lack of inserts on the basis of the sacB gene is known as:

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Q. F (fertility) factor is used in many bacterial systems for different purposes. Choose the incorrect statement with respect to the F factor.

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Q. F factor integration into the host chromosome is called as __________

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Q. Excision of the F factor can also be done from the host chromosome. Choose the incorrect statement in respect to it.

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Q. The low copy number of F plasmid is essential for the stability of these plasmids.

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Q. Choose the correct statement for BAC vector system.

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Q. The bacteriophage M13 vectors belong to a group of phages called as __________

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Q. Choose the correct statement for the infection process of M13.

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Q. Once the bacteriophage enters the bacterial cells, some changes are carried out in it. Which of the following is not included?

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Q. Replication can also be carried out by rolling circle replication. Choose the correct statement for this type of replication?

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Q. Gene V blocks the production of which gene?

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Q. What is the size of the DNA molecule which can be packed by the viral protein coat?

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Q. The uniform layer of cells is known as lawn and the holes peppered from infected cells are termed as __________

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Q. M13 doesn’t actually lyse the cells.

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Q. The plaques formed by M13 infection are called as __________

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Q. M13 vector is tightly packed. Where is the available site to insert DNA?

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