Q. What is the basis of RNaseH method?

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Q. The first step for RNaseH method is to anneal a chemically synthesized oligo-dT primer to the 3’ polyA tail of RNA.

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Q. For the synthesis of the second DNA strand, incorporated oligo-dT is of no use. Why?

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Q. Choose the incorrect statement for the homopolymer tailing of cDNA strands.

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Q. Choose the correct statement if the RNA is non polydenylated.

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Q. In case if molecules smaller than the fragments required for making a full genomic library are used for making a collection. This collection is called as ___________

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Q. Choose the correct statement for construction of a library subsection.

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Q. Any cDNA library would represent a fraction of RNA species of an organism.

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Q. What do we mean by housekeeping genes?

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Q. Choose the correct statement for RNA fractionation.

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Q. What is done after RNA fractionation is carried out?

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Q. The polypeptides produced after addition of mRNA are analysed with antibodies. Choose the incorrect statement for this analysis.

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Q. What is done after the recovery of pellets has been carried out in order to know the amount of polypeptides?

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Q. Sometimes a gene which we want to clone is present on a particular chromosome. For this purpose, the chromosome should be in which phase?

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Q. For cloning purposes, the intact chromosomes should be separated by ___________

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Q. The process of examining stained chromosomes in a light microscope and removing appropriate regions with a micro-manipulator is called as ___________

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Q. If the library to be screened and the host belongs to different species, then it is called as ___________

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Q. The direct selection allows _______ number of recombinants to be screened and _______

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Q. Xenopus oocyte cells are also used at the time to carry out screening. Choose the incorrect statement for this procedure.

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Q. In the case of ligand binding by the expressed protein, the library can be screened by ______________

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