Q. What is done after RNA fractionation is carried out?

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Q. The polypeptides produced after addition of mRNA are analysed with antibodies. Choose the incorrect statement for this analysis.

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Q. What is done after the recovery of pellets has been carried out in order to know the amount of polypeptides?

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Q. Sometimes a gene which we want to clone is present on a particular chromosome. For this purpose, the chromosome should be in which phase?

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Q. For cloning purposes, the intact chromosomes should be separated by ___________

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Q. The process of examining stained chromosomes in a light microscope and removing appropriate regions with a micro-manipulator is called as ___________

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Q. If the library to be screened and the host belongs to different species, then it is called as ___________

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Q. The direct selection allows _______ number of recombinants to be screened and _______

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Q. Xenopus oocyte cells are also used at the time to carry out screening. Choose the incorrect statement for this procedure.

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Q. In the case of ligand binding by the expressed protein, the library can be screened by ______________

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Q. In the case of immunochemical screening, the position of ________ antibody is detected by ________ antibody.

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Q. The ligand should bind to a single protein only for the screening process.

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Q. Choose the incorrect statement for panning.

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Q. If screening is carried out by using a combination of nascent peptide and mRNA, then it is called as ________

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Q. Libraries can broadly be classified into how many types?

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Q. Choose the correct statement for genomic libraries.

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Q. Choose the incorrect statement for the preparation of genomic libraries.

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Q. The various steps for construction of libraries are ___________

i) Fragmentation of DNA
ii) Isolation of genomic DNA
iii) Amplification
iv) Ligation and introduction to the host
v) Vector preparation

The correct order of construction of libraries is (In the order of starting to ending).

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Q. Complete digestion is preferred over partial digestion for the fragmentation of DNA.

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Q. To avoid ligation of separate DNA fragments, which of the enzyme is used?

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