Question

Choose the incorrect statement for the preparation of genomic libraries.

a.

The first step is the isolation of genomic DNA

b.

Physical damage to the DNA should be avoided

c.

If a nuclear DNA library is to be constructed, organelle DNA is to be removed

d.

For the construction of organelle library, organelle DNA is purified from the nuclear DNA

Posted under Genetic Engineering

Answer: (c).If a nuclear DNA library is to be constructed, organelle DNA is to be removed

Interact with the Community - Share Your Thoughts

Uncertain About the Answer? Seek Clarification Here.

Understand the Explanation? Include it Here.

Q. Choose the incorrect statement for the preparation of genomic libraries.

Similar Questions

Explore Relevant Multiple Choice Questions (MCQs)

Q. The various steps for construction of libraries are ___________

i) Fragmentation of DNA
ii) Isolation of genomic DNA
iii) Amplification
iv) Ligation and introduction to the host
v) Vector preparation

The correct order of construction of libraries is (In the order of starting to ending).

Q. Complete digestion is preferred over partial digestion for the fragmentation of DNA.

Q. To avoid ligation of separate DNA fragments, which of the enzyme is used?

Q. Vector and insert are mixed, ligated and packaged and introduced into the host by ___________

Q. Libraries using phage cloning vectors are often kept as ___________

Q. Libraries constructed in plasmid vectors can be kept as ___________

Q. During storage, there are chances of degradation of DNA. Larger fragments are having more chances of being got lost.

Q. Choose the incorrect statement for cDNA libraries.

Q. A times partial sequencing of cloned cDNAs is carried out. These cDNA are known as ___________

Q. Polyadenylation of RNA species is an important criterion for the production of cDNA species. Which of the following holds true?

Q. Choose the incorrect statement for oligo-dT cellulose.

Q. Poly A tail from the column is eluted by using high salt concentration.

Q. Choose the incorrect statement for second DNA strand synthesis.

Q. What is the final product of the RNaseH method?

Q. What would not happen if the RNA strand is completely removed from RNA: DNA hybrid?

Q. The loop region is single stranded. It can be cleaved by using which enzyme?

Q. Choose the correct statement with respect to the self priming method of cDNA synthesis.

Q. Choose the incorrect statement for the method homopolymer tailing.

Q. Choose the correct statement for RACE.

Q. The first primer in the case of 3’ RACE is ___________

Recommended Subjects

Are you eager to expand your knowledge beyond Genetic Engineering? We've handpicked a range of related categories that you might find intriguing.

Click on the categories below to discover a wealth of MCQs and enrich your understanding of various subjects. Happy exploring!