Q. Libraries constructed in plasmid vectors can be kept as ___________

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Q. During storage, there are chances of degradation of DNA. Larger fragments are having more chances of being got lost.

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Q. Choose the incorrect statement for cDNA libraries.

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Q. A times partial sequencing of cloned cDNAs is carried out. These cDNA are known as ___________

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Q. Polyadenylation of RNA species is an important criterion for the production of cDNA species. Which of the following holds true?

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Q. Choose the incorrect statement for oligo-dT cellulose.

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Q. Poly A tail from the column is eluted by using high salt concentration.

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Q. Choose the incorrect statement for second DNA strand synthesis.

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Q. What is the final product of the RNaseH method?

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Q. What would not happen if the RNA strand is completely removed from RNA: DNA hybrid?

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Q. The loop region is single stranded. It can be cleaved by using which enzyme?

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Q. Choose the correct statement with respect to the self priming method of cDNA synthesis.

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Q. Choose the incorrect statement for the method homopolymer tailing.

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Q. Choose the correct statement for RACE.

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Q. The first primer in the case of 3’ RACE is ___________

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Q. The first cDNA strand in 5’ RACE is tailed with oligo-dA tail.

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Q. What is the second primer in the case of 5’ RACE?

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Q. RNaseH method and homopolymer tailing method generates blunt ended cDNA molecules. Which of the following can be used for attaching them to vector?

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Q. Choose the correct statement for modification of homopolymer tailing method.

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Q. By synthesizing two strands separately then annealing them leads to formation of double stranded oligonucleotide.

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