Q. The first cDNA strand in 5’ RACE is tailed with oligo-dA tail.

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Q. What is the second primer in the case of 5’ RACE?

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Q. RNaseH method and homopolymer tailing method generates blunt ended cDNA molecules. Which of the following can be used for attaching them to vector?

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Q. Choose the correct statement for modification of homopolymer tailing method.

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Q. By synthesizing two strands separately then annealing them leads to formation of double stranded oligonucleotide.

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Q. Sometimes the required mRNA is present in less number. So the process of increasing the representation of rare mRNAs is called as ____________

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Q. Choose the correct statement in order to enrich rare species.

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Q. Which statement holds true for hydroxyapatite?

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Q. Libraries in which a particular sequence is present in one organism but are absent from another organism, are called as ___________

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Q. The nucleic acid from the cell type that contains the sequence we are interested in is called ___________

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Q. Choose the correct statement for libraries constructed by mixing cell types differing in the sequences they are having.

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Q. Which of the following is desirable?

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Q. Which of the statement holds true?

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Q. If physical methods are used, tracer is amplified and is labelled with biotin.

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Q. The process of finding a particular member of the library which is having some defined properties is called as ___________

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Q. If for a particular organism sequence data is available and we have to simply search in the data through a computer, then this method is called as ___________

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Q. If we are having sequence data for a particular organism, but screening is carried out for homologues the program used is BLAST.

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Q. If screening is carried out on the basis of sequences which are related to the desired sequence, then the process is called ___________

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Q. How many techniques are there for carrying out the screening of sequences encoding for RNAs?

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Q. Choose the incorrect statement for colony or plaque lift.

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