Q. The screening of libraries us carried out by nucleic acid hybridisation and constitutes of following steps:

i) Peeling of membranes carrying away bacterial cells with it
ii) Cells are lysed and denaturing of DNA is being carried out
iii) Hybridization with labelled DNA
iv) Placing the membrane onto plate containing recombinant cells

Choose the correct sequence in which the steps are carried out (starting to ending).

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Q. Choose the incorrect statement with respect to the membranes used for adhering of bacterial cells onto them.

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Q. Nitrocellulose membranes are less sensitive than nylon membranes.

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Q. What is used for lysing of bacterial cells and denaturation of DNA?

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Q. The process of baking or cross-linking of the membrane is carried out by ___________

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Q. Choose the incorrect statement for prehybridization mixture.

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Q. After hybridization how the position of the labelled DNA probe is determined?

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Q. The important parameters for precise annealing of the probe and the subsequent washing don’t include ___________

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Q. If hybridization is carried out with a clone having a sequence similar to that of the vector from which library has been constructed, then it is of no use. Choose the correct statement in respect to it.

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Q. At what temperatures the hybridization and washing of the DNA probes should be carried out?

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Q. Which library should be used, genomic or cDNA when the screening is carried out by oligonucleotide probes?

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Q. Sometimes successive rounds of screening of a genomic library are carried out and an ordered collection of clones is done in a linear fashion, then the process is called as ___________

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Q. If the clone for a gene is obtained on the basis of its position in the gene map, then it is called as ___________

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Q. Mobile portions of a chromosome which can be transferred from one portion to another are termed as ___________

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Q. At times screening is done for the protein product of DNA of interest rather than the sequence itself. How many methods are there to carry out this?

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Q. Choose the correct statement if the screening is carried out by screening by expression in vivo.

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Q. Choose the incorrect statement with respect to host used.

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Q. If the gene to be screened belongs to prokaryotic species then there are chances that it is not expressed.

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Q. If an expression of the coding sequence is to be carried in the vitro, then which of the statement holds true?

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Q. If translation and transcription are taking place together, the reaction is called as ______ and if they are carried out separately, it is called as _______

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