Question

If an expression of the coding sequence is to be carried in the vitro, then which of the statement holds true?

a.

The extract in which the host DNA constructs are incubated is capable of translation only

b.

The extract in which the host DNA constructs are incubated is capable of transcription only

c.

The extract is made up of lysate of E. coli cells, containing RNA polymerase, ribosomes, tRNAs etc

d.

Non radiolabelled amino acids are used

Answer: (c).The extract is made up of lysate of E. coli cells, containing RNA polymerase, ribosomes, tRNAs etc

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Q. If an expression of the coding sequence is to be carried in the vitro, then which of the statement holds true?

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