Q. For two hybrid systems, activation domains can be present in different proteins also rather than being on a single protein. A sequence encodes _______ protein for which we want to find an interacting protein.

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Q. In two hybrid screening system, the activator binds through _______ domain to a sequence upstream of the gene under its control, and ________ domain stimulates transcription.

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Q. Screening is often carried out for a sequence that interacts with a protein for which already a clone is present. It is carried out in which host?

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Q. Sequences that can function as origins of replication are called as ___________

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Q. In case of promoter-probe vectors, the same or related species should be as a vector whose DNA is to be screened.

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Q. Luciferase genes are also used at times for detection. Choose the correct statement for them.

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Q. GFP is one of a marker which is used for screening libraries in hosts other than E. coli. Choose the incorrect statement for GFP.

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Q. Promoter-probe vectors are used often. Choose the correct statement for these vectors.

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Q. In ______ organisms, firstly the gene is looked into model organisms.

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Q. For identification of genes that are expressed under special conditions, _______ libraries are used.

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Q. Choose the correct statement with respect to the bacterial genome.

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Q. If the genome size is small, then sequencing of the entire genome is ___________

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Q. Introns are ________ in nature and the sequence is _______ than exons.

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Q. Choose the incorrect statement for the open reading frame.

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Q. If the screening of the DNA constructs is carried out in pooled batches then it takes less time.

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Q. If translation and transcription are carried out separately, then what is added to carry out translation?

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Q. If translation and transcription are taking place together, the reaction is called as ______ and if they are carried out separately, it is called as _______

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Q. If an expression of the coding sequence is to be carried in the vitro, then which of the statement holds true?

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Q. If the gene to be screened belongs to prokaryotic species then there are chances that it is not expressed.

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Q. Choose the incorrect statement with respect to host used.

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