Q. Nitrocellulose membranes are less sensitive than nylon membranes.

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Q. What is used for lysing of bacterial cells and denaturation of DNA?

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Q. The process of baking or cross-linking of the membrane is carried out by ___________

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Q. Choose the incorrect statement for prehybridization mixture.

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Q. After hybridization how the position of the labelled DNA probe is determined?

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Q. The important parameters for precise annealing of the probe and the subsequent washing don’t include ___________

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Q. If hybridization is carried out with a clone having a sequence similar to that of the vector from which library has been constructed, then it is of no use. Choose the correct statement in respect to it.

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Q. At what temperatures the hybridization and washing of the DNA probes should be carried out?

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Q. Which library should be used, genomic or cDNA when the screening is carried out by oligonucleotide probes?

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Q. Sometimes successive rounds of screening of a genomic library are carried out and an ordered collection of clones is done in a linear fashion, then the process is called as ___________

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Q. If the clone for a gene is obtained on the basis of its position in the gene map, then it is called as ___________

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Q. Mobile portions of a chromosome which can be transferred from one portion to another are termed as ___________

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Q. At times screening is done for the protein product of DNA of interest rather than the sequence itself. How many methods are there to carry out this?

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Q. Choose the correct statement if the screening is carried out by screening by expression in vivo.

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Q. Choose the incorrect statement with respect to host used.

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Q. If the gene to be screened belongs to prokaryotic species then there are chances that it is not expressed.

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Q. If an expression of the coding sequence is to be carried in the vitro, then which of the statement holds true?

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Q. If translation and transcription are taking place together, the reaction is called as ______ and if they are carried out separately, it is called as _______

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Q. If translation and transcription are carried out separately, then what is added to carry out translation?

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Q. If the screening of the DNA constructs is carried out in pooled batches then it takes less time.

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