Q. What is the second primer in the case of 5’ RACE?

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Q. RNaseH method and homopolymer tailing method generates blunt ended cDNA molecules. Which of the following can be used for attaching them to vector?

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Q. Choose the correct statement for modification of homopolymer tailing method.

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Q. By synthesizing two strands separately then annealing them leads to formation of double stranded oligonucleotide.

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Q. Sometimes the required mRNA is present in less number. So the process of increasing the representation of rare mRNAs is called as ____________

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Q. Choose the correct statement in order to enrich rare species.

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Q. Which statement holds true for hydroxyapatite?

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Q. Libraries in which a particular sequence is present in one organism but are absent from another organism, are called as ___________

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Q. The nucleic acid from the cell type that contains the sequence we are interested in is called ___________

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Q. Choose the correct statement for libraries constructed by mixing cell types differing in the sequences they are having.

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Q. Which of the following is desirable?

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Q. Which of the statement holds true?

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Q. If physical methods are used, tracer is amplified and is labelled with biotin.

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Q. The process of finding a particular member of the library which is having some defined properties is called as ___________

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Q. If for a particular organism sequence data is available and we have to simply search in the data through a computer, then this method is called as ___________

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Q. If we are having sequence data for a particular organism, but screening is carried out for homologues the program used is BLAST.

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Q. If screening is carried out on the basis of sequences which are related to the desired sequence, then the process is called ___________

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Q. How many techniques are there for carrying out the screening of sequences encoding for RNAs?

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Q. Choose the incorrect statement for colony or plaque lift.

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Q. The screening of libraries us carried out by nucleic acid hybridisation and constitutes of following steps:

i) Peeling of membranes carrying away bacterial cells with it
ii) Cells are lysed and denaturing of DNA is being carried out
iii) Hybridization with labelled DNA
iv) Placing the membrane onto plate containing recombinant cells

Choose the correct sequence in which the steps are carried out (starting to ending).

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