Q. If physical methods are used, tracer is amplified and is labelled with biotin.

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Q. The process of finding a particular member of the library which is having some defined properties is called as ___________

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Q. If for a particular organism sequence data is available and we have to simply search in the data through a computer, then this method is called as ___________

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Q. If we are having sequence data for a particular organism, but screening is carried out for homologues the program used is BLAST.

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Q. If screening is carried out on the basis of sequences which are related to the desired sequence, then the process is called ___________

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Q. How many techniques are there for carrying out the screening of sequences encoding for RNAs?

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Q. Choose the incorrect statement for colony or plaque lift.

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Q. The screening of libraries us carried out by nucleic acid hybridisation and constitutes of following steps:

i) Peeling of membranes carrying away bacterial cells with it
ii) Cells are lysed and denaturing of DNA is being carried out
iii) Hybridization with labelled DNA
iv) Placing the membrane onto plate containing recombinant cells

Choose the correct sequence in which the steps are carried out (starting to ending).

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Q. Choose the incorrect statement with respect to the membranes used for adhering of bacterial cells onto them.

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Q. Nitrocellulose membranes are less sensitive than nylon membranes.

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Q. What is used for lysing of bacterial cells and denaturation of DNA?

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Q. The process of baking or cross-linking of the membrane is carried out by ___________

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Q. Choose the incorrect statement for prehybridization mixture.

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Q. After hybridization how the position of the labelled DNA probe is determined?

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Q. The important parameters for precise annealing of the probe and the subsequent washing don’t include ___________

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Q. If hybridization is carried out with a clone having a sequence similar to that of the vector from which library has been constructed, then it is of no use. Choose the correct statement in respect to it.

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Q. At what temperatures the hybridization and washing of the DNA probes should be carried out?

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Q. Which library should be used, genomic or cDNA when the screening is carried out by oligonucleotide probes?

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Q. Sometimes successive rounds of screening of a genomic library are carried out and an ordered collection of clones is done in a linear fashion, then the process is called as ___________

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Q. If the clone for a gene is obtained on the basis of its position in the gene map, then it is called as ___________

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