Question

Polyadenylation of RNA species is an important criterion for the production of cDNA species. Which of the following holds true?

a.

Polyadenylation should be at 3’ end

b.

Eukaryotic mRNAs are mostly non-polyadenylated

c.

Bacterial mRNAs and organelle mRNAs are polyadenylated

d.

It is carried out by the addition of T residues after synthesis

Answer: (a).Polyadenylation should be at 3’ end

Interact with the Community - Share Your Thoughts

Uncertain About the Answer? Seek Clarification Here.

Understand the Explanation? Include it Here.

Q. Polyadenylation of RNA species is an important criterion for the production of cDNA species. Which of the following holds true?

Similar Questions

Explore Relevant Multiple Choice Questions (MCQs)

Q. Choose the incorrect statement for oligo-dT cellulose.

Q. Poly A tail from the column is eluted by using high salt concentration.

Q. Choose the incorrect statement for second DNA strand synthesis.

Q. What is the final product of the RNaseH method?

Q. What would not happen if the RNA strand is completely removed from RNA: DNA hybrid?

Q. The loop region is single stranded. It can be cleaved by using which enzyme?

Q. Choose the correct statement with respect to the self priming method of cDNA synthesis.

Q. Choose the incorrect statement for the method homopolymer tailing.

Q. Choose the correct statement for RACE.

Q. The first primer in the case of 3’ RACE is ___________

Q. The first cDNA strand in 5’ RACE is tailed with oligo-dA tail.

Q. What is the second primer in the case of 5’ RACE?

Q. RNaseH method and homopolymer tailing method generates blunt ended cDNA molecules. Which of the following can be used for attaching them to vector?

Q. Choose the correct statement for modification of homopolymer tailing method.

Q. By synthesizing two strands separately then annealing them leads to formation of double stranded oligonucleotide.

Q. Sometimes the required mRNA is present in less number. So the process of increasing the representation of rare mRNAs is called as ____________

Q. Choose the correct statement in order to enrich rare species.

Q. Which statement holds true for hydroxyapatite?

Q. Libraries in which a particular sequence is present in one organism but are absent from another organism, are called as ___________

Q. The nucleic acid from the cell type that contains the sequence we are interested in is called ___________

Recommended Subjects

Are you eager to expand your knowledge beyond Genetic Engineering? We've handpicked a range of related categories that you might find intriguing.

Click on the categories below to discover a wealth of MCQs and enrich your understanding of various subjects. Happy exploring!