Question

Choose the correct statement with respect to the self priming method of cDNA synthesis.

a.

It is less preferred than RNaseH method

b.

A hairpin structure is formed with guarantee

c.

The sequence corresponding to the 5’ end is lost

d.

Reverse transcriptase is not used

Posted under Genetic Engineering

Answer: (c).The sequence corresponding to the 5’ end is lost

Interact with the Community - Share Your Thoughts

Uncertain About the Answer? Seek Clarification Here.

Understand the Explanation? Include it Here.

Q. Choose the correct statement with respect to the self priming method of cDNA synthesis.

Similar Questions

Explore Relevant Multiple Choice Questions (MCQs)

Q. Choose the incorrect statement for the method homopolymer tailing.

Q. Choose the correct statement for RACE.

Q. The first primer in the case of 3’ RACE is ___________

Q. The first cDNA strand in 5’ RACE is tailed with oligo-dA tail.

Q. What is the second primer in the case of 5’ RACE?

Q. RNaseH method and homopolymer tailing method generates blunt ended cDNA molecules. Which of the following can be used for attaching them to vector?

Q. Choose the correct statement for modification of homopolymer tailing method.

Q. By synthesizing two strands separately then annealing them leads to formation of double stranded oligonucleotide.

Q. Sometimes the required mRNA is present in less number. So the process of increasing the representation of rare mRNAs is called as ____________

Q. Choose the correct statement in order to enrich rare species.

Q. Which statement holds true for hydroxyapatite?

Q. Libraries in which a particular sequence is present in one organism but are absent from another organism, are called as ___________

Q. The nucleic acid from the cell type that contains the sequence we are interested in is called ___________

Q. Choose the correct statement for libraries constructed by mixing cell types differing in the sequences they are having.

Q. Which of the following is desirable?

Q. Which of the statement holds true?

Q. If physical methods are used, tracer is amplified and is labelled with biotin.

Q. The process of finding a particular member of the library which is having some defined properties is called as ___________

Q. If for a particular organism sequence data is available and we have to simply search in the data through a computer, then this method is called as ___________

Q. If we are having sequence data for a particular organism, but screening is carried out for homologues the program used is BLAST.

Recommended Subjects

Are you eager to expand your knowledge beyond Genetic Engineering? We've handpicked a range of related categories that you might find intriguing.

Click on the categories below to discover a wealth of MCQs and enrich your understanding of various subjects. Happy exploring!