Q. A times partial sequencing of cloned cDNAs is carried out. These cDNA are known as ___________

View solution

Q. Polyadenylation of RNA species is an important criterion for the production of cDNA species. Which of the following holds true?

View solution

Q. Choose the incorrect statement for oligo-dT cellulose.

View solution

Q. Poly A tail from the column is eluted by using high salt concentration.

View solution

Q. Choose the incorrect statement for second DNA strand synthesis.

View solution

Q. What is the final product of the RNaseH method?

View solution

Q. What would not happen if the RNA strand is completely removed from RNA: DNA hybrid?

View solution

Q. The loop region is single stranded. It can be cleaved by using which enzyme?

View solution

Q. Choose the correct statement with respect to the self priming method of cDNA synthesis.

View solution

Q. Choose the incorrect statement for the method homopolymer tailing.

View solution

Q. Choose the correct statement for RACE.

View solution

Q. The first primer in the case of 3’ RACE is ___________

View solution

Q. The first cDNA strand in 5’ RACE is tailed with oligo-dA tail.

View solution

Q. What is the second primer in the case of 5’ RACE?

View solution

Q. RNaseH method and homopolymer tailing method generates blunt ended cDNA molecules. Which of the following can be used for attaching them to vector?

View solution

Q. Choose the correct statement for modification of homopolymer tailing method.

View solution

Q. By synthesizing two strands separately then annealing them leads to formation of double stranded oligonucleotide.

View solution

Q. Sometimes the required mRNA is present in less number. So the process of increasing the representation of rare mRNAs is called as ____________

View solution

Q. Choose the correct statement in order to enrich rare species.

View solution

Q. Which statement holds true for hydroxyapatite?

View solution