Q. If a putative protein sequence is cloned in an expression vector and the expressed protein is not showing protease activity, then which of the following is not correct?

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Q. For getting a large amount of proteins to crystallize, which of the following should be used as an expression system?

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Q. If a mutation perturbs the structure, then stability and folding are not affected.

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Q. The RNA level ___________ the steady-state level of the corresponding protein directly and the post-translational modification of the protein ____________

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Q. For a convenient transformation system, _____ can be used for gene silencing.

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Q. The term ‘endonuclease’ refers to cutting the DNA sequence from ______________

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Q. The restriction endonuclease is having a defence mechanism in the bacterial system against foreign DNA such as viruses. But how it is able to protect its own DNA?

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Q. Even after replication, how the modified DNA remains protected?

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Q. How many classes of restriction enzymes are there?

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Q. Type II cuts the sequence in the following way __________

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Q. After cleaving the sequence, the nature of the ends created by the type II endonuclease is __________

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Q. A sequence is having two ends, 5’ and 3’. Which of the following statements is correct regarding the nature of the ends?

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Q. Blunt ends created by the restriction endonuclease can be joined.

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Q. The recognition sequence for BamHI is 5’ G|GATCC 3’. The ‘|’ represents the cutting site. What can be inferred about the ends from it?

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Q. The recognition sequence of Sau3A is 5’ |GATC 3’ and that for DpnI is 5’ GA|TC 3’. Which of the statements is true?

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Q. The recognition sequence is at times palindromic in nature. Which of the following statements is correct with respect to it?

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Q. If all the nucleotides are present with equal frequencies and at random, what are the chances of having a particular four nucleotide long motif?

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Q. Isoschizomers are defined as ___________

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Q. Isocaudomers are defined as the enzymes which recognize different sequence but generate same ends. Which of the following pairs of enzymes can be termed as isocuadomers?

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Q. The specificity of an enzyme is affected by the concentration of buffer used. This phenomenon is termed as:

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