Q. After cleaving the sequence, the nature of the ends created by the type II endonuclease is __________

View solution

Q. A sequence is having two ends, 5’ and 3’. Which of the following statements is correct regarding the nature of the ends?

View solution

Q. Blunt ends created by the restriction endonuclease can be joined.

View solution

Q. The recognition sequence for BamHI is 5’ G|GATCC 3’. The ‘|’ represents the cutting site. What can be inferred about the ends from it?

View solution

Q. The recognition sequence of Sau3A is 5’ |GATC 3’ and that for DpnI is 5’ GA|TC 3’. Which of the statements is true?

View solution

Q. The recognition sequence is at times palindromic in nature. Which of the following statements is correct with respect to it?

View solution

Q. If all the nucleotides are present with equal frequencies and at random, what are the chances of having a particular four nucleotide long motif?

View solution

Q. Isoschizomers are defined as ___________

View solution

Q. Isocaudomers are defined as the enzymes which recognize different sequence but generate same ends. Which of the following pairs of enzymes can be termed as isocuadomers?

View solution

Q. The specificity of an enzyme is affected by the concentration of buffer used. This phenomenon is termed as:

View solution

Q. Which of the following statements is correct with respect to a unit of an enzyme?

View solution

Q. Which of the following statement is correct regarding partial digestion?

View solution

Q. Which of the following is the correct nomenclature of a restriction enzyme obtained from the first activity of strain R of Escherichia coli?

View solution

Q. Why is DNase preferred over restriction endonuclease in some cases?

View solution

Q. The ends created by use of DNase have unique single stranded sequences.

View solution

Q. Besides enzymatic means, physical stress can also be used for cleaving the DNA. Which of the following statements is true?

View solution

Q. Phosphatases refer to __________

View solution

Q. How is phosphatase related to the ligation reactions?

View solution

Q. How can phosphatase activity terminated prior to a ligation reaction?

View solution

Q. Basic classification of polymerases includes how many types?

View solution