Q. Why is DNase preferred over restriction endonuclease in some cases?

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Q. The ends created by use of DNase have unique single stranded sequences.

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Q. Besides enzymatic means, physical stress can also be used for cleaving the DNA. Which of the following statements is true?

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Q. Phosphatases refer to __________

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Q. How is phosphatase related to the ligation reactions?

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Q. How can phosphatase activity terminated prior to a ligation reaction?

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Q. Basic classification of polymerases includes how many types?

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Q. Polymerase can be defined as ______________

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Q. Which of the following activity is not possible in the case of DNA polymerase I?

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Q. The E. coli DNA polymerase enzyme gives different domains with different activities on cleaving with protease subtilisin. Which of the following statements is correct with respect to the fragments generated?

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Q. Removal of single stranded portions produced due to exonucleolytic activity and due to polymerase activity are termed as _____________

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Q. Thermostable DNA polymerases are very important in PCR. How are they obtained?

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Q. Which of the following enzyme is said as reverse transcriptase?

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Q. Why reverse transcriptase enzymes are having comparatively high error rates than other polymerases?

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Q. Which polymerase can be used in conjunction with appropriate phage promoters in order to have high levels of specific transcription?

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Q. Template independent polymerases are the enzymes which add nucleotide bases without a template. Which of the following statements is correct with respect to these?

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Q. Which of the following statements is correct with respect to exonuclease?

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Q. How many approaches are there which can be used for exonucleolytic activity in double stranded DNA molecules?

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Q. What is the mode of action of exonuclease III?

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Q. Which of the following statements is correct regarding S1 nuclease?

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