Q. Which polymerase can be used in conjunction with appropriate phage promoters in order to have high levels of specific transcription?

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Q. Template independent polymerases are the enzymes which add nucleotide bases without a template. Which of the following statements is correct with respect to these?

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Q. Which of the following statements is correct with respect to exonuclease?

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Q. How many approaches are there which can be used for exonucleolytic activity in double stranded DNA molecules?

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Q. What is the mode of action of exonuclease III?

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Q. Which of the following statements is correct regarding S1 nuclease?

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Q. What happens if a DNA molecule is treated by first Exonuclease III and then followed by treatment with S1 nuclease?

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Q. How can one end be protected from the action of Exonuclease III, so that the molecule is not shortened from both the ends?

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Q. Bal31 is also an enzyme which is used. Which of the following statements hold true in its context?

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Q. The extent of deletions can be manipulated by controlling which of them?

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Q. What is the function of methylase?

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Q. Methylase is useful in cloning experiments.

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Q. Ligation is defined as ______________

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Q. If only one bond is broken in the sugar-phosphate backbone, it is called as ___________

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Q. How many categories of ligation reaction are there on the basis of ends created?

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Q. In the case of blunt-end ligation, blunt ends can be generated by ___________

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Q. The ligation reaction is more efficient in which case?

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Q. The sticky ends are held together by which type of bonds?

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Q. Why sticky ended ligations are carried out at temperatures lower than room temperature?

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Q. Ligation reaction can be both intramolecular and intermolecular in nature.

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