Question
a.
Alignment of only double stranded DNA molecules at the ends and the formation of phosphodiester bonds between both the strands
b.
Alignment of either of the double or single stranded DNA molecules and formation of glycosidic bonds between both the strands
c.
Alignment of either of the double or single stranded DNA molecules and the formation of phosphodiester bonds. The bond can be between either one or both the strands
d.
Alignment of single stranded DNA molecules and formation of glycosidic bonds between these strands
Posted under Basics of Genetic Engineering Genetic Engineering
Interact with the Community - Share Your Thoughts
Uncertain About the Answer? Seek Clarification Here.
Understand the Explanation? Include it Here.
Q. Ligation is defined as ______________
Similar Questions
Explore Relevant Multiple Choice Questions (MCQs)
Q. If only one bond is broken in the sugar-phosphate backbone, it is called as ___________
View solution
Q. How many categories of ligation reaction are there on the basis of ends created?
View solution
Q. In the case of blunt-end ligation, blunt ends can be generated by ___________
View solution
Q. The ligation reaction is more efficient in which case?
View solution
Q. The sticky ends are held together by which type of bonds?
View solution
Q. Why sticky ended ligations are carried out at temperatures lower than room temperature?
View solution
Q. Ligation reaction can be both intramolecular and intermolecular in nature.
View solution
Q. If a ligation reaction is being carried out and recircularization is observed, which type of reaction is being carried out?
View solution
Q. What is the kinetics of the intramolecular and intermolecular ligation reactions?
View solution
Q. What are the effects of increasing concentration of reaction components?
View solution
Q. Enzyme commonly used for carrying out ligation reaction is ____________
View solution
Q. Which of the following statements is correct with respect to T4 DNA ligase?
View solution
Q. If blunt ended ligations are to be carried out. Which of the following enzymes can be used?
View solution
Q. E. coli DNA ligase doesn’t require a cofactor.
View solution
Q. Mechanism of ligation for both T4 DNA ligase and E. coli DNA ligase makes use of Adenosine Monophosphate (AMP). Which of the steps is involved in the ligation mechanism?
View solution
Q. Topoisomerase is also an enzyme which is used for carrying out ligation. The correct statement for topoisomerase is?
View solution
Q. Mobile genetic elements can be transferred from one DNA portion to another. The enzyme carrying out this is ____________
View solution
Q. Phage based recombination systems are used for ____________
View solution
Q. Bacteriophage lambda is having a phage recombination system. Following are the characteristics of this system ____________
View solution
Q. Ligation enzymes are used for ligating newly synthesized okazaki fragments. What holds true for okazaki fragments?
View solution
Recommended Subjects
Are you eager to expand your knowledge beyond Genetic Engineering? We've handpicked a range of related categories that you might find intriguing.
Click on the categories below to discover a wealth of MCQs and enrich your understanding of various subjects. Happy exploring!
