Question
a.
AMP is added to the 5’ phosphate of one of the DNA molecule
b.
It leads to the liberation of pyrophosphate from NAD and nicotinamide mononucleotide from ATP
c.
The AMP is further displaced by an electrophilic attack
d.
The AMP is further displaced by nucleophilic attack by 3’ hydroxyl of the same DNA molecule
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Q. Mechanism of ligation for both T4 DNA ligase and E. coli DNA ligase makes use of Adenosine Monophosphate (AMP). Which of the steps is involved in the ligation mechanism?
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