Q. Plasmids can be classified into how many types depending on the genes present for their transformation?

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Q. Viral infection can also be used to take up the DNA by the cells.

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Q. Chemical transformation refers to the methods which use chemicals in order to carry out transformation. Which of the following statements is true with respect to it?

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Q. Electroporation is also used for taking up the DNA by the cells. It constitutes of ____________

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Q. Transformation carried out using a particle gun is known as biolistic transformation. It falls under which category of transformation?

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Q. The particle gun method consists of which of the following steps?

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Q. Isolation of genomic DNA follows the same principles as that of obtaining plasmid from E. coli. Which of the following is not included in it?

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Q. How many methods are there for obtaining the plasmid DNA from the bacteria?

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Q. Cell lysis is carried out by which substance?

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Q. Chromosomal or genomic DNA is separated by ____________

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Q. Proteins can be removed via treatment by?

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Q. The nucleic acid remaining in the solution can be precipitated by addition of sodium or ammonium acetate and ethanol.

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Q. Gel electrophoresis separates nucleic acid molecules based on ____________

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Q. The charge present on the DNA backbone is negative. The force required to accelerate the molecules towards anode is directly proportional to the number of ____________

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Q. Force is defined as mass per unit acceleration. As the number of phosphate molecules increases, the charge also increases which increases the force required. The acceleration is dependent on the size of the molecules.

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Q. A piece of DNA that is to be separated is crushed and soaked into the buffer. The majority of DNA diffuses, how can it be separated?

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Q. Another method of DNA recovery is termed as freeze-squeeze. The correct statement for it is?

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Q. If further electrophoresis is used for recovery of DNA from gels, the method is termed as ____________

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Q. The DNA is sliced and is placed into the dialysis tube containing buffer. Further electrophoresis is performed. After it, the DNA moves out of the gel but is retained by buffer.

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Q. Often the DNA or RNA needs to be removed from the gel to the solid support. The process can be termed as ____________

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