Q. How many categories of ligation reaction are there on the basis of ends created?

View solution

Q. In the case of blunt-end ligation, blunt ends can be generated by ___________

View solution

Q. The ligation reaction is more efficient in which case?

View solution

Q. The sticky ends are held together by which type of bonds?

View solution

Q. Why sticky ended ligations are carried out at temperatures lower than room temperature?

View solution

Q. Ligation reaction can be both intramolecular and intermolecular in nature.

View solution

Q. If a ligation reaction is being carried out and recircularization is observed, which type of reaction is being carried out?

View solution

Q. What is the kinetics of the intramolecular and intermolecular ligation reactions?

View solution

Q. What are the effects of increasing concentration of reaction components?

View solution

Q. Enzyme commonly used for carrying out ligation reaction is ____________

View solution

Q. Which of the following statements is correct with respect to T4 DNA ligase?

View solution

Q. If blunt ended ligations are to be carried out. Which of the following enzymes can be used?

View solution

Q. E. coli DNA ligase doesn’t require a cofactor.

View solution

Q. Mechanism of ligation for both T4 DNA ligase and E. coli DNA ligase makes use of Adenosine Monophosphate (AMP). Which of the steps is involved in the ligation mechanism?

View solution

Q. Topoisomerase is also an enzyme which is used for carrying out ligation. The correct statement for topoisomerase is?

View solution

Q. Mobile genetic elements can be transferred from one DNA portion to another. The enzyme carrying out this is ____________

View solution

Q. Phage based recombination systems are used for ____________

View solution

Q. Bacteriophage lambda is having a phage recombination system. Following are the characteristics of this system ____________

View solution

Q. Ligation enzymes are used for ligating newly synthesized okazaki fragments. What holds true for okazaki fragments?

View solution

Q. Introduction of DNA molecules into the recipient organism is termed as ____________

View solution