Question
a.
It is so because the vibrational and kinetic energy of the molecules at room temperature is lower than that of the energy required to break the bonds holding the ends
b.
The energy required to break the bonds holding the ends is very less than that of the kinetic and vibrational energy at room temperature
c.
The enzyme carrying out ligation is unstable at low temperature
d.
The sticky ends created, don’t just relegate at low temperature
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Q. Why sticky ended ligations are carried out at temperatures lower than room temperature?
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