Q. Mechanism of ligation for both T4 DNA ligase and E. coli DNA ligase makes use of Adenosine Monophosphate (AMP). Which of the steps is involved in the ligation mechanism?

View solution

Q. Topoisomerase is also an enzyme which is used for carrying out ligation. The correct statement for topoisomerase is?

View solution

Q. Mobile genetic elements can be transferred from one DNA portion to another. The enzyme carrying out this is ____________

View solution

Q. Phage based recombination systems are used for ____________

View solution

Q. Bacteriophage lambda is having a phage recombination system. Following are the characteristics of this system ____________

View solution

Q. Ligation enzymes are used for ligating newly synthesized okazaki fragments. What holds true for okazaki fragments?

View solution

Q. Introduction of DNA molecules into the recipient organism is termed as ____________

View solution

Q. Many bacterial species are having a natural ability to take up the exogenous DNA material. Which of the statement is not correct in regard to it?

View solution

Q. Which of the statements hold true for conjugation?

View solution

Q. F plasmid is often used in conjugation. The correct statement is?

View solution

Q. Plasmids can be classified into how many types depending on the genes present for their transformation?

View solution

Q. Viral infection can also be used to take up the DNA by the cells.

View solution

Q. Chemical transformation refers to the methods which use chemicals in order to carry out transformation. Which of the following statements is true with respect to it?

View solution

Q. Electroporation is also used for taking up the DNA by the cells. It constitutes of ____________

View solution

Q. Transformation carried out using a particle gun is known as biolistic transformation. It falls under which category of transformation?

View solution

Q. The particle gun method consists of which of the following steps?

View solution

Q. Isolation of genomic DNA follows the same principles as that of obtaining plasmid from E. coli. Which of the following is not included in it?

View solution

Q. How many methods are there for obtaining the plasmid DNA from the bacteria?

View solution

Q. Cell lysis is carried out by which substance?

View solution

Q. Chromosomal or genomic DNA is separated by ____________

View solution