Q. E. coli DNA ligase doesn’t require a cofactor.

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Q. Mechanism of ligation for both T4 DNA ligase and E. coli DNA ligase makes use of Adenosine Monophosphate (AMP). Which of the steps is involved in the ligation mechanism?

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Q. Topoisomerase is also an enzyme which is used for carrying out ligation. The correct statement for topoisomerase is?

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Q. Mobile genetic elements can be transferred from one DNA portion to another. The enzyme carrying out this is ____________

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Q. Phage based recombination systems are used for ____________

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Q. Bacteriophage lambda is having a phage recombination system. Following are the characteristics of this system ____________

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Q. Ligation enzymes are used for ligating newly synthesized okazaki fragments. What holds true for okazaki fragments?

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Q. Introduction of DNA molecules into the recipient organism is termed as ____________

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Q. Many bacterial species are having a natural ability to take up the exogenous DNA material. Which of the statement is not correct in regard to it?

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Q. Which of the statements hold true for conjugation?

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Q. F plasmid is often used in conjugation. The correct statement is?

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Q. Plasmids can be classified into how many types depending on the genes present for their transformation?

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Q. Viral infection can also be used to take up the DNA by the cells.

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Q. Chemical transformation refers to the methods which use chemicals in order to carry out transformation. Which of the following statements is true with respect to it?

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Q. Electroporation is also used for taking up the DNA by the cells. It constitutes of ____________

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Q. Transformation carried out using a particle gun is known as biolistic transformation. It falls under which category of transformation?

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Q. The particle gun method consists of which of the following steps?

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Q. Isolation of genomic DNA follows the same principles as that of obtaining plasmid from E. coli. Which of the following is not included in it?

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Q. How many methods are there for obtaining the plasmid DNA from the bacteria?

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Q. Cell lysis is carried out by which substance?

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