Q. How can one end be protected from the action of Exonuclease III, so that the molecule is not shortened from both the ends?

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Q. Bal31 is also an enzyme which is used. Which of the following statements hold true in its context?

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Q. The extent of deletions can be manipulated by controlling which of them?

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Q. What is the function of methylase?

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Q. Methylase is useful in cloning experiments.

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Q. Ligation is defined as ______________

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Q. If only one bond is broken in the sugar-phosphate backbone, it is called as ___________

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Q. How many categories of ligation reaction are there on the basis of ends created?

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Q. In the case of blunt-end ligation, blunt ends can be generated by ___________

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Q. The ligation reaction is more efficient in which case?

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Q. The sticky ends are held together by which type of bonds?

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Q. Why sticky ended ligations are carried out at temperatures lower than room temperature?

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Q. Ligation reaction can be both intramolecular and intermolecular in nature.

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Q. If a ligation reaction is being carried out and recircularization is observed, which type of reaction is being carried out?

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Q. What is the kinetics of the intramolecular and intermolecular ligation reactions?

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Q. What are the effects of increasing concentration of reaction components?

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Q. Enzyme commonly used for carrying out ligation reaction is ____________

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Q. Which of the following statements is correct with respect to T4 DNA ligase?

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Q. If blunt ended ligations are to be carried out. Which of the following enzymes can be used?

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Q. E. coli DNA ligase doesn’t require a cofactor.

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