Question

How can one end be protected from the action of Exonuclease III, so that the molecule is not shortened from both the ends?

a.

By using Phosphorothioate nucleotide analogue

b.

By making both the ends double stranded in nature

c.

By labelling one end with a radioactive compound

d.

By increasing the time of exposure of the DNA molecule to the enzyme

Posted under Genetic Engineering

Answer: (a).By using Phosphorothioate nucleotide analogue

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Q. How can one end be protected from the action of Exonuclease III, so that the molecule is not shortened from both the ends?

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