Q. How many approaches are there which can be used for exonucleolytic activity in double stranded DNA molecules?

View solution

Q. What is the mode of action of exonuclease III?

View solution

Q. Which of the following statements is correct regarding S1 nuclease?

View solution

Q. What happens if a DNA molecule is treated by first Exonuclease III and then followed by treatment with S1 nuclease?

View solution

Q. How can one end be protected from the action of Exonuclease III, so that the molecule is not shortened from both the ends?

View solution

Q. Bal31 is also an enzyme which is used. Which of the following statements hold true in its context?

View solution

Q. The extent of deletions can be manipulated by controlling which of them?

View solution

Q. What is the function of methylase?

View solution

Q. Methylase is useful in cloning experiments.

View solution

Q. Ligation is defined as ______________

View solution

Q. If only one bond is broken in the sugar-phosphate backbone, it is called as ___________

View solution

Q. How many categories of ligation reaction are there on the basis of ends created?

View solution

Q. In the case of blunt-end ligation, blunt ends can be generated by ___________

View solution

Q. The ligation reaction is more efficient in which case?

View solution

Q. The sticky ends are held together by which type of bonds?

View solution

Q. Why sticky ended ligations are carried out at temperatures lower than room temperature?

View solution

Q. Ligation reaction can be both intramolecular and intermolecular in nature.

View solution

Q. If a ligation reaction is being carried out and recircularization is observed, which type of reaction is being carried out?

View solution

Q. What is the kinetics of the intramolecular and intermolecular ligation reactions?

View solution

Q. What are the effects of increasing concentration of reaction components?

View solution