Question
a.
The smaller fragment is having C terminal and the larger fragment is having N terminal
b.
The smaller fragment is named as Klenow fragment and the intact molecule is called as Kornberg fragment
c.
The smaller fragment is having 5’-3’ exonuclease activity whereas the larger fragment is having 5’-3’ polymerase and 3’-5’ exonuclease activity
d.
Both the fragments are having 5’-3’ polymerase and 3’-5’ exonuclease activity
Posted under Genetic Engineering
Interact with the Community - Share Your Thoughts
Uncertain About the Answer? Seek Clarification Here.
Understand the Explanation? Include it Here.
Q. The E. coli DNA polymerase enzyme gives different domains with different activities on cleaving with protease subtilisin. Which of the following statements is correct with respect...
Similar Questions
Explore Relevant Multiple Choice Questions (MCQs)
Q. Removal of single stranded portions produced due to exonucleolytic activity and due to polymerase activity are termed as _____________
View solution
Q. Thermostable DNA polymerases are very important in PCR. How are they obtained?
View solution
Q. Which of the following enzyme is said as reverse transcriptase?
View solution
Q. Why reverse transcriptase enzymes are having comparatively high error rates than other polymerases?
View solution
Q. Which polymerase can be used in conjunction with appropriate phage promoters in order to have high levels of specific transcription?
View solution
Q. Template independent polymerases are the enzymes which add nucleotide bases without a template. Which of the following statements is correct with respect to these?
View solution
Q. Which of the following statements is correct with respect to exonuclease?
View solution
Q. How many approaches are there which can be used for exonucleolytic activity in double stranded DNA molecules?
View solution
Q. What is the mode of action of exonuclease III?
View solution
Q. Which of the following statements is correct regarding S1 nuclease?
View solution
Q. What happens if a DNA molecule is treated by first Exonuclease III and then followed by treatment with S1 nuclease?
View solution
Q. How can one end be protected from the action of Exonuclease III, so that the molecule is not shortened from both the ends?
View solution
Q. Bal31 is also an enzyme which is used. Which of the following statements hold true in its context?
View solution
Q. The extent of deletions can be manipulated by controlling which of them?
View solution
Q. What is the function of methylase?
View solution
Q. Methylase is useful in cloning experiments.
View solution
Q. Ligation is defined as ______________
View solution
Q. If only one bond is broken in the sugar-phosphate backbone, it is called as ___________
View solution
Q. How many categories of ligation reaction are there on the basis of ends created?
View solution
Q. In the case of blunt-end ligation, blunt ends can be generated by ___________
View solution
Recommended Subjects
Are you eager to expand your knowledge beyond Genetic Engineering? We've handpicked a range of related categories that you might find intriguing.
Click on the categories below to discover a wealth of MCQs and enrich your understanding of various subjects. Happy exploring!
