Q. Molecules having new combination of sequences that were not present before are called as __________

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Q. Insertion of DNA into lacZ gene may lead to disruption of the gene function.

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Q. Which of the following is true regarding taking up of plasmid DNA in the bacterial cells?

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Q. If genomic DNA has been inserted instead of the plasmid, what will happen?

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Q. Often PCR can be performed in order to confirm whether an insert is present in the plasmid. Cells are taken directly and PCR is performed, this type of PCR is known as __________

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Q. Generation of recombinants by randomly cloning fragments of total DNA from an organism is called as __________

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Q. The phenomenon of alpha complementation is __________

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Q. An operon is defined as __________

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Q. Why the whole lacZ gene can’t be present in the plasmid at one time?

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Q. Alpha complementation is an indicator of lacZ system. Which of the statement is incorrect for it?

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Q. What will be the consequence of not having an origin of replication (ori) in the vector?

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Q. It is required to distinguish between the cells that have taken up the vector and that have not. It is done by using __________

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Q. Multiple cloning site (MCS) is defined as __________

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Q. Size of the vector is related to having a suitable single restriction site.

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Q. If a restriction site is 6 nucleotides long, what are the chances of finding it in a vector?

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Q. Which of the statement is true for pBR322?

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Q. If a plasmid is having two antibiotic resistant genes, say ampicillin resistant and chloramphenicol resistant. If the plasmid grows in ampicillin containing medium but not in chloramphenicol, what can be concluded?

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Q. If high copy number is there, the replication is called as ______ and if low copy number is there the replication is called as ______

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Q. Chloramphenicol amplification is carried out in various plasmids. Which of the statement is incorrect for it?

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Q. The phenomenon of not allowing the recombinants to escape into the environment is termed as __________

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