Q. Insertion of DNA into lacZ gene may lead to disruption of the gene function.

View solution

Q. Which of the following is true regarding taking up of plasmid DNA in the bacterial cells?

View solution

Q. If genomic DNA has been inserted instead of the plasmid, what will happen?

View solution

Q. Often PCR can be performed in order to confirm whether an insert is present in the plasmid. Cells are taken directly and PCR is performed, this type of PCR is known as __________

View solution

Q. Generation of recombinants by randomly cloning fragments of total DNA from an organism is called as __________

View solution

Q. The phenomenon of alpha complementation is __________

View solution

Q. An operon is defined as __________

View solution

Q. Why the whole lacZ gene can’t be present in the plasmid at one time?

View solution

Q. Alpha complementation is an indicator of lacZ system. Which of the statement is incorrect for it?

View solution

Q. What will be the consequence of not having an origin of replication (ori) in the vector?

View solution

Q. It is required to distinguish between the cells that have taken up the vector and that have not. It is done by using __________

View solution

Q. Multiple cloning site (MCS) is defined as __________

View solution

Q. Size of the vector is related to having a suitable single restriction site.

View solution

Q. If a restriction site is 6 nucleotides long, what are the chances of finding it in a vector?

View solution

Q. Which of the statement is true for pBR322?

View solution

Q. If a plasmid is having two antibiotic resistant genes, say ampicillin resistant and chloramphenicol resistant. If the plasmid grows in ampicillin containing medium but not in chloramphenicol, what can be concluded?

View solution

Q. If high copy number is there, the replication is called as ______ and if low copy number is there the replication is called as ______

View solution

Q. Chloramphenicol amplification is carried out in various plasmids. Which of the statement is incorrect for it?

View solution

Q. The phenomenon of not allowing the recombinants to escape into the environment is termed as __________

View solution

Q. How will we choose which transformation method is to be used?

View solution