Question

What happens if insert DNA is cut with two different restriction enzymes at the ends?

a.

It is difficult to insert the fragment

b.

The insert can be ligated in any orientation

c.

The insert can be ligated in one orientation only

d.

The amount of product increases

Answer: (c).The insert can be ligated in one orientation only

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Q. What happens if insert DNA is cut with two different restriction enzymes at the ends?

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