Q. The gene to be disrupted is cloned and a selectable marker is inserted. What should be the effect of selectable marker?

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Q. The disrupted gene is excised from the vector and is inserted into the target organism. The excised gene should be in which form?

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Q. Stable acquisition of the marker can take place only if a double crossover over the flanking sequence and their chromosomal counterparts causes the marker’s integration into the chromosome.

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Q. If ______ gene is there, the double crossover may leave ________ in the chromosome.

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Q. If the target organism contains more than one copy of the gene, what is the effect on these copies?

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Q. If methods are based on cellular processes that lead to inactivation of gene expression by affecting the RNA, then it is called as ___________

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Q. Choose the incorrect statement for the method based on antisense RNA.

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Q. The method of post transcriptional gene silencing is particularly useful in ___________

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Q. Down regulation of expression of endogenous genes by transformation with constructs that would generate sense RNA, rather than anti-sense RNA is known as ___________

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Q. In some organisms, presence of double stranded RNAs leads to breakdown of corresponding single stranded mRNA.

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Q. Double stranded RNA is cleaved by a nuclease called as Dicer and small fragments are generated known as ___________

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Q. The process of RNA inactivation by siRNAs is termed as ___________

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Q. DNA _________ is also a method for gene silencing through short RNAs.

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Q. siRNAs can either be introduced directly or by microinjection.

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Q. Introduction into host organism can also be done by using a DNA construct, which when transcribed, generates an RNA which is _________

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Q. The ________ protein of bacteriophage P1 mediates site-specific recombination at a 34 bp sequence, loxP.

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Q. Choose the incorrect statement for cre-lox excision.

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Q. Cre can be introduced by crossing it with a strain containing the gene or by infection with virus containing it.

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Q. How much effect is there on the surrounding genes by the loxP sequence which is left after recombination has taken place?

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Q. The ability to control the expression of cre allows controlling what?

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