Q. Capping can be introduced by the use of cap analogue. Which of the statement is true?

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Q. If transcription should not be carried out beyond the insert in the vector, then it should be linearized.

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Q. If the cells containing plasmids are infected with helper phage, which type of DNA can be produced, packaged and secreted into the medium?

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Q. Little quantities of radiolabelled proteins are required for which of the following?

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Q. __________ quantities of _______ protein are required for determination of properties in biochemical and biological assays.

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Q. Small quantities of radiolabelled RNA can be produced by translation in vitro which is done by transcription in vitro.

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Q. For the production of unlabelled and huge amount of proteins, which of the following is true?

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Q. How many methods are there, which are used for protein synthesis in vitro?

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Q. Reticulocytes are immature red cells, which are obtained from ___________

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Q. What is the use of adding micrococcal nuclease in the reticulocyte cells?

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Q. Treatment of reticulocyte cells is done with EGTA. It chelates the calcium ions which are required for functioning of micrococcal nuclease.

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Q. In ________ transcription and translation is coupled.

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Q. In which of the following systems, transcription and translation are carried out together?

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Q. If overexpression of proteins takes place, they become toxic at times. In such a case _______ colonies are recovered after ______ with the recombinant plasmid.

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Q. Ribosome binding site in E.coli is composed of which sequence primarily?

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Q. Translation starts at first _____ codon in the mRNA downstream from the ribosome binding site.

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Q. Expression of cloned sequences in E.coli requires a promoter, a ribosome binding site and an initiation codon.

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Q. If an expression vector contains all three, viz. a controllable promoter, a ribosome binding site and an initiation codon, then a hybrid protein is produced. The _______ region of hybrid protein is encoded by vector and the rest is expressed by the sequence inserted.

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Q. Proteins synthesized by carrying out a translation of the vector region and continuing it in the insert region is called as _____________

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Q. It is also possible to generate a fusion protein in which ______ is encoded by the vector.

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