Q. If overexpression of proteins takes place, they become toxic at times. In such a case _______ colonies are recovered after ______ with the recombinant plasmid.

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Q. Ribosome binding site in E.coli is composed of which sequence primarily?

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Q. Translation starts at first _____ codon in the mRNA downstream from the ribosome binding site.

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Q. Expression of cloned sequences in E.coli requires a promoter, a ribosome binding site and an initiation codon.

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Q. If an expression vector contains all three, viz. a controllable promoter, a ribosome binding site and an initiation codon, then a hybrid protein is produced. The _______ region of hybrid protein is encoded by vector and the rest is expressed by the sequence inserted.

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Q. Proteins synthesized by carrying out a translation of the vector region and continuing it in the insert region is called as _____________

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Q. It is also possible to generate a fusion protein in which ______ is encoded by the vector.

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Q. Which of the following is present in E. coli rrnB operon for rRNA?

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Q. Termination codon is required for stopping high levels of transcription of other regions such as _______ which might interfere with ___________ of the vector.

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Q. In fusion proteins, the reading frame in a vector is out of phase with the insert.

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Q. Some vectors carry a mutant form of promoter known as lacUV5 promoter. It carries ______ in the promoter region and _____ the efficiency.

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Q. Lambda PL promoter is used in which vectors?

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Q. The promoter can be controlled by a repressor which is temperature sensitive.

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Q. Choose the correct statement for rifampcin.

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Q. The ______ operon encodes proteins involved in arabinose metabolism.

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Q. tac promoter is an example of which type of promoter?

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Q. The tac promoter is made by _____ region of trp promoter and _____ region of the lacUV5 promoter.

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Q. The tac promoter includes the lac operator and is regulated by a repressor.

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Q. Expression of T7 promoter- lac operator hybrid requires ___________

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Q. When lacUV5 control system is used, addition of IPTG _______ the expression of _________

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