Q. Termination codon is required for stopping high levels of transcription of other regions such as _______ which might interfere with ___________ of the vector.

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Q. In fusion proteins, the reading frame in a vector is out of phase with the insert.

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Q. Some vectors carry a mutant form of promoter known as lacUV5 promoter. It carries ______ in the promoter region and _____ the efficiency.

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Q. Lambda PL promoter is used in which vectors?

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Q. The promoter can be controlled by a repressor which is temperature sensitive.

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Q. Choose the correct statement for rifampcin.

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Q. The ______ operon encodes proteins involved in arabinose metabolism.

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Q. tac promoter is an example of which type of promoter?

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Q. The tac promoter is made by _____ region of trp promoter and _____ region of the lacUV5 promoter.

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Q. The tac promoter includes the lac operator and is regulated by a repressor.

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Q. Expression of T7 promoter- lac operator hybrid requires ___________

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Q. When lacUV5 control system is used, addition of IPTG _______ the expression of _________

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Q. There are some advantages of expressing protein as a fusion protein. It may enhance stability, folding ______ and ______ formation.

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Q. A short peptide region fused to a protein of interest is known as ___________

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Q. Glutathione-S-Transferase (GST) enzyme is used for the conjunction of Glutathione molecules and is having a protective function in many organisms.

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Q. If it is desirable to express two or more proteins simultaneously, then it is known as ___________

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Q. For co-expression of proteins, it is possible to have several independent sets of promoters and other signals in a single vector.

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Q. A considerable amount of modification can take place in the ______ region of RNA in _______ before translation. It affects the yield of proteins.

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Q. Strong secondary structure in a message may affect ________

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Q. What is the function of T7 lysozyme?

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