Q. Secretion of overexpressed proteins by fusion to a secretion signal can be useful because it helps in bringing about correct _______ and _____ protein folding.

View solution

Q. The enzyme used for breaking down of sucrose into glucose and fructose is called as _______________

View solution

Q. For introduction of exogenous DNA into S.cerevisiae ______ is added along with polyethylene glycol and heat shock treatment.

View solution

Q. Protoplast transformation is less time consuming than lithium acetate for transformation.

View solution

Q. Schizosaccharomyces pombe is yeast and it reproduces by __________

View solution

Q. The leu1+ marker encodes which enzyme?

View solution

Q. The replacement of one plasmid by another by introduction of the second plasmid into strain containing the first, followed by removal of the first by counterselection is known as __________

View solution

Q. Promoters can be divided basically into how many types?

View solution

Q. nmt1+ gene is used for _____ biosynthesis.

View solution

Q. The promoter from fbp1+ gene is suppressed in the presence of glucose.

View solution

Q. Species which can grow on _______ as a carbon source are called as methylotropic.

View solution

Q. Chlamyodomonas reinhardtii is a green _________ algae.

View solution

Q. Chlamyodomonas genome is _______ rich.

View solution

Q. Integration of DNA into the Chlamydomonas genome is via homologous recombination.

View solution

Q. The disease crown gall is caused by which bacteria?

View solution

Q. Agrobacterium tumefaciens form ______ plasmids and Agrobacterium rhizogenes form _______ plasmids.

View solution

Q. The region which is transferred from bacterium to the nucleus of the plant cell is called as T-DNA.

View solution

Q. Transfer of T DNA depends on a set of genes called as ______ if they are present on ________

View solution

Q. What is the function of onc genes in TDNA?

View solution

Q. Which of the plant growth regulators are produced by TDNA?

View solution