Q. A wide range of host cell lines are available and commonly used human cell lines are obtained from ___________

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Q. DEAE-dextran is used for introduction of DNA. It is a modified _____ and is ______

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Q. Cells whose contents have been removed and replaced, by swelling and shrinking in solutions of suitable osmotic strength are called as ____________

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Q. In ___________ cells, virus replication doesn’t take place and viral DNA ___________

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Q. SV40 is a virus and it produces how many transcripts?

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Q. Splicing of the transcripts is necessary for efficient expression.

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Q. T protein is required for replication. It is a product of ____________

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Q. Vaccinia virus is a commonly used virus and it is a member of pox virus family. It has a large genome of _____________ and can accept at least ____________ of foreign DNA.

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Q. Vaccinia virus produces its own ______ which is functional in _____ of infected cells where the virus also replicates.

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Q. It is beneficial to use retrovirus for genetic manipulation of cells in the culture.

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Q. LNSX is a retrovirus and is combined with ______ in order to create a shuttle vector.

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Q. The retroviral transfected DNA is infected into mammalian cells by helper retrovirus. Helper retrovirus should not be packaged and the method adopted for this is?

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Q. Choose the incorrect statement in respect to MACs.

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Q. Centromere is the most important component of the human artificial chromosome. It is constructed from tandemly repeated copies of 171 bp of ______ DNA sequence.

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Q. Constitutive promoters are majorly obtained from ________

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Q. In naturally occurring bacterial systems, tetracycline binding repressor protein binds in the _____ of tetracycline to the control sequence and _______ transcription.

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Q. Tetracycline binding repressor protein when fused to transcription activator protein from herpes simple virus, then it is called as transactivator protein (tTA).

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Q. Expression of tTA gene is ________ by doxycycline which is _______ analogue.

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Q. Glucorticoid-responsive element is ______ promoter from ______ of mouse mammory tumour virus (MMTV).

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Q. There are a group of promoters from the genes for metallothioneins. These are a group of proteins rich in ______ residues.

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