Q. It is beneficial to use retrovirus for genetic manipulation of cells in the culture.

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Q. LNSX is a retrovirus and is combined with ______ in order to create a shuttle vector.

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Q. The retroviral transfected DNA is infected into mammalian cells by helper retrovirus. Helper retrovirus should not be packaged and the method adopted for this is?

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Q. Choose the incorrect statement in respect to MACs.

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Q. Centromere is the most important component of the human artificial chromosome. It is constructed from tandemly repeated copies of 171 bp of ______ DNA sequence.

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Q. Constitutive promoters are majorly obtained from ________

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Q. In naturally occurring bacterial systems, tetracycline binding repressor protein binds in the _____ of tetracycline to the control sequence and _______ transcription.

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Q. Tetracycline binding repressor protein when fused to transcription activator protein from herpes simple virus, then it is called as transactivator protein (tTA).

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Q. Expression of tTA gene is ________ by doxycycline which is _______ analogue.

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Q. Glucorticoid-responsive element is ______ promoter from ______ of mouse mammory tumour virus (MMTV).

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Q. There are a group of promoters from the genes for metallothioneins. These are a group of proteins rich in ______ residues.

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Q. The amount of expression of DNA inserted into a vector _______ on promoter and ______ on chromosomal location.

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Q. If the embryo is at one-cell stage then it is found in ____________

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Q. Embryonic stem cells are also used for generation of transgenic organisms. They are obtained from _______ of a developing ________

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Q. Embryonic stem cells (ES) are isolated and are injected again into the blastocoel of a developing embryo. The embryo which develops is entirely made up of these cells only.

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Q. ES cells are used in order to ensure that insertion is done at the required chromosomal location and it is called as ____________

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Q. If a gene is inactivated by gene targeting then it is called as ____________

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Q. Integration events may be insertional involving _____ crossover or replacement involving _____ crossovers.

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Q. It is necessary to analyze the genome of transgenic cells to confirm that the site of integration is correct. This is carried out by southern blotting.

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Q. The gene targeting approach produces individuals which are ______ for inactivation of the gene.

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