Q. Which of the following is true about restriction endonucleases?

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Q. Which of the following enzymes’ combined action leads to the generation of sticky ends in the plasmid vector?

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Q. Generally a plasmid vector contains how many elements?

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Q. To make the recombinant plasmid permeable to DNA molecules, which of the chemicals is added?

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Q. Which of the following statements is false?

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Q. The extra chromosomal, self-replicating, closed, double stranded and circular DNA molecule is generally termed as __________

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Q. E.cor1 is a __________

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Q. In which stage of genetic engineering a probe is used?

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Q. What is a DNA library?

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Q. Which of the following enzyme is required for end to end joining of DNA?

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Q. Repressor molecules bind to __________

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Q. Generally, YAC contains how many elements?

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Q. Which of the following endonuclease removes a length of DNA between two telomere sequences?

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Q. In hybridization method, which of the following is pressed onto agar?

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Q. Which of the following temperature best suits for the growth and multiplication of transformed cells?

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Q. Which of the following is a DNA element that instructs RNA polymerase where to bind on DNA?

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Q. Site directed mutagenesis facilitated research on __________

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Q. Recombinant DNA molecule is produced by ligating __________

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Q. Arrange the following steps involved in Gene cloning in the correct order.

i) Isolation of desired gene
ii) Insertion of isolated gene to the vector
iii) Introduction of recombinant vector to the host
iv) Expression of recombinant gene in host
v) Extraction of recombinant gene product

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Q. Where does a restriction endonuclease cuts DNA?

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