Q. Once the proteins are translocated within the organelles, protease cleavage takes place to remove the signal sequences and generate mature proteins.

View solution

Q. The signal sequences have a _________ consensus but contain some specific features. They all have a ______ core region preceded by one or more positively charged residues.

View solution

Q. The signal sequences are typically _______ residues long, rich in _____ charged residues such as arginines as well as hydroxyl residues such as serines and threonines, but devoid of ______ charged residues.

View solution

Q. Chloroplast localization signals are also located in the __________ terminus and are about 25 to 100 residues in length, containing very few _______ charged residues but many hydroxylated residues such as serine.

View solution

Q. Chloroplast localization signals consist of two adjacent signal peptides, one for targeting the proteins to the stromaportion of the chloroplast before being cleaved and the other for targeting the remaining portion of the proteins to the thylakoids.

View solution

Q. Which of the following is an incorrect statement about SignalP?

View solution

Q. Which of the following is not one of the training sets in SignalP?

View solution

Q. TargetP is a neural network-based program, similar to SignalP.

View solution

Q. Which of the following is untrue regarding the classic yeast two-hybrid method?

View solution

Q. If the bait and prey proteins _______ they bring the DNA-binding and trans-activation domains in such close proximity that they reconstitute the function of the transcription activator, turning ____ the expression of a reporter gene as a result. Which of the following is not the correct pair of blanks?

View solution

Q. Which of the following is untrue regarding the classic yeast two-hybrid method?

View solution

Q. An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column.

View solution

Q. Which of the following is untrue regarding the Predicting Interactions Based on Domain Fusion?

View solution

Q. When the two domains are located in two different proteins, to preserve the same functionality, their close proximity and interaction have to be preserved as well.

View solution

Q. The justification behind Rosetta stone method is that when two domains are fused in a single protein, they have to be in _______ proximity to perform a common function.

View solution

Q. In Predicting Interactions Based on Gene Neighbors if a certain gene linkage is found to be indeed conserved across divergent genomes, it can be used as a strong indicator of formation of an operon that encodes proteins that are functionally and even physically coupled.

View solution

Q. Which of the following is untrue regarding the predicting Interactions Based on Phylogenetic Information?

View solution

Q. Which of the following is untrue regarding the STRING?

View solution