Q. An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column.

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Q. Which of the following is untrue regarding the classic yeast two-hybrid method?

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Q. If the bait and prey proteins _______ they bring the DNA-binding and trans-activation domains in such close proximity that they reconstitute the function of the transcription activator, turning ____ the expression of a reporter gene as a result. Which of the following is not the correct pair of blanks?

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Q. Which of the following is untrue regarding the classic yeast two-hybrid method?

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Q. TargetP is a neural network-based program, similar to SignalP.

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Q. Which of the following is not one of the training sets in SignalP?

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Q. Which of the following is an incorrect statement about SignalP?

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Q. Chloroplast localization signals consist of two adjacent signal peptides, one for targeting the proteins to the stromaportion of the chloroplast before being cleaved and the other for targeting the remaining portion of the proteins to the thylakoids.

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Q. Chloroplast localization signals are also located in the __________ terminus and are about 25 to 100 residues in length, containing very few _______ charged residues but many hydroxylated residues such as serine.

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Q. The signal sequences are typically _______ residues long, rich in _____ charged residues such as arginines as well as hydroxyl residues such as serines and threonines, but devoid of ______ charged residues.

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Q. The signal sequences have a _________ consensus but contain some specific features. They all have a ______ core region preceded by one or more positively charged residues.

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Q. Once the proteins are translocated within the organelles, protease cleavage takes place to remove the signal sequences and generate mature proteins.

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Q. For many eukaryotic proteins, newly synthesized protein precursors have to be transported to specific membrane-bound compartments and be proteolytically processed to become functional.

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Q. Which of the following is an incorrect statement about the terminologies related to protein sorting?

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Q. ExPASY contains a number of programs to determine posttranslational modifications based on MS molecular mass data.

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Q. Cysteine doesn’t make predictions by building profiles.

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Q. Only Advanced neural networks are used to discern long-distance pairwise interactions among cysteine residues.

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Q. Accurate prediction of _____ bonds may also help to predict the _____ dimensional structure of the protein of interest.

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Q. A disulfide bridge is a unique type of _____ modification in which _____ bonds are formed between cysteine residues.

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Q. In a statistical learning process called support vector machine (SVM), a hyperplane is _____

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