Q. ExPASY contains a number of programs to determine posttranslational modifications based on MS molecular mass data.

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Q. Which of the following is an incorrect statement about the terminologies related to protein sorting?

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Q. For many eukaryotic proteins, newly synthesized protein precursors have to be transported to specific membrane-bound compartments and be proteolytically processed to become functional.

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Q. Once the proteins are translocated within the organelles, protease cleavage takes place to remove the signal sequences and generate mature proteins.

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Q. The signal sequences have a _________ consensus but contain some specific features. They all have a ______ core region preceded by one or more positively charged residues.

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Q. The signal sequences are typically _______ residues long, rich in _____ charged residues such as arginines as well as hydroxyl residues such as serines and threonines, but devoid of ______ charged residues.

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Q. Chloroplast localization signals are also located in the __________ terminus and are about 25 to 100 residues in length, containing very few _______ charged residues but many hydroxylated residues such as serine.

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Q. Chloroplast localization signals consist of two adjacent signal peptides, one for targeting the proteins to the stromaportion of the chloroplast before being cleaved and the other for targeting the remaining portion of the proteins to the thylakoids.

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Q. Which of the following is an incorrect statement about SignalP?

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Q. Which of the following is not one of the training sets in SignalP?

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Q. TargetP is a neural network-based program, similar to SignalP.

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Q. Which of the following is untrue regarding the classic yeast two-hybrid method?

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Q. If the bait and prey proteins _______ they bring the DNA-binding and trans-activation domains in such close proximity that they reconstitute the function of the transcription activator, turning ____ the expression of a reporter gene as a result. Which of the following is not the correct pair of blanks?

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Q. Which of the following is untrue regarding the classic yeast two-hybrid method?

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Q. An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column.

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Q. Which of the following is untrue regarding the Predicting Interactions Based on Domain Fusion?

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Q. When the two domains are located in two different proteins, to preserve the same functionality, their close proximity and interaction have to be preserved as well.

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Q. The justification behind Rosetta stone method is that when two domains are fused in a single protein, they have to be in _______ proximity to perform a common function.

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Q. In Predicting Interactions Based on Gene Neighbors if a certain gene linkage is found to be indeed conserved across divergent genomes, it can be used as a strong indicator of formation of an operon that encodes proteins that are functionally and even physically coupled.

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Q. Which of the following is untrue regarding the predicting Interactions Based on Phylogenetic Information?

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