Transcription in Prokaryotes and Eukaryotes MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Transcription in Prokaryotes and Eukaryotes, a fundamental topic in the field of Cytogenetics. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Transcription in Prokaryotes and Eukaryotes MCQs are designed to help you grasp the core concepts and excel in solving problems.

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Transcription in Prokaryotes and Eukaryotes MCQs | Page 8 of 16

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Q71.
Prokaryotes have 1 RNA polymerase. How many RNA polymerases are there in eukaryotes?

a.

2

b.

3

c.

4

d.

None

Discuss
Answer: (b).3
Q72.
You isolate the nucleolus from the nucleus and performed a density gradient centrifugation with the extract. Then you check the different for the RNA polymerase activity of the different segment. Which RNA polymerase would you expect to find in abundance?

a.

RNA pol I

b.

RNA pol II

c.

RNA pol III

d.

RNA pol IV

Discuss
Answer: (a).RNA pol I
Q73.
Which RNA polymerase can bind CBP?

a.

I

b.

II

c.

III

d.

None

Discuss
Answer: (b).II
Q74.
What is the sequence of the heptad repeat of C terminal domain?

a.

TSPWSTT

b.

YSPTSGS

c.

YSPTSPS

d.

YSLPSTS

Discuss
Answer: (c).YSPTSPS
Q75.
You designed an antibody against phosphorylated CTD of RNA polymerase II. Then you use a DNA fragment with lac operon and try to immunoprecipitate the RNA pol II. What will be the expected result?

a.

Moderate concentration of RNA pol II will precipitate

b.

RNA pol II that precipitated will be contaminated with pol III

c.

High amount of RNA pol II will precipitate

d.

No RNA pol II will precipitate

Discuss
Answer: (d).No RNA pol II will precipitate
Q76.
If you consider a transcribing RNA pol II with a transcript as small as 30 bases, which serine residue in its CTD will be phosphorylated?

a.

2

b.

5

c.

7

d.

None

Discuss
Answer: (b).5
Q77.
Which factor phosphorylates the serine residues in RNA pol II CTD?

a.

TFIIA

b.

TFIIB

c.

TFIIH

d.

TFIID

Discuss
Answer: (c).TFIIH
Q78.
In what sequence are the serine residues in RNA pol II phosphorylated?

a.

5->2->7

b.

2->5->7

c.

2->7->5

d.

7->2->5

Discuss
Answer: (c).2->7->5
Q79.
In an experiment you add growing concentration of the alpha amanitin. What will be the sequence in which RNA polymerases are affected if at all?

a.

Pol I -> pol III , pol II unaffected

b.

pol I->pol I ->pol III

c.

pol III->pol II -> pol I

d.

pol II -> pol III , pol I unaffected

Discuss
Answer: (d).pol II -> pol III , pol I unaffected
Q80.
RPA 2 is one of the larger subunits of RNA polymerase in eukaryotes. It is homologous to the prokaryotic _____________

a.

Alpha subunit

b.

Beta subunit

c.

Beta prime subunit

d.

Omega subunit

Discuss
Answer: (b).Beta subunit
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