Enzyme Assay and Enzyme Catalysis MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Enzyme Assay and Enzyme Catalysis, a fundamental topic in the field of Enzyme Technology. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Enzyme Assay and Enzyme Catalysis MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Enzyme Assay and Enzyme Catalysis mcq questions that explore various aspects of Enzyme Assay and Enzyme Catalysis problems. Each MCQ is crafted to challenge your understanding of Enzyme Assay and Enzyme Catalysis principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Enzyme Technology tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Enzyme Assay and Enzyme Catalysis MCQs are your pathway to success in mastering this essential Enzyme Technology topic.

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Enzyme Assay and Enzyme Catalysis MCQs | Page 3 of 4

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Q21.
In which of the following methods, the intensity of emitted light is used to study enzyme reaction?

a.

Discontinuous assay

b.

Luminescence method

c.

Biosensors

d.

Spectrophotometer

Discuss
Answer: (c).Biosensors
Q22.
Continuous assay: Glucose-6-phosphate dehydrogenase:: Luminescence method: ____________

a.

Hydrolases

b.

Bacterial luciferase

c.

Ornithine decarboxylase

d.

Glutamate decarboxylase

Discuss
Answer: (b).Bacterial luciferase
Q23.
Which of the following is not true for luminescence method?

a.

The intensity of emitted light is used to study enzyme reaction

b.

The reaction can be used to assay ATP and appropriate enzymes via coupled reaction

c.

Bacterial luciferase can be assayed and measured by this method

d.

It is based on stoke shift

Discuss
Answer: (d).It is based on stoke shift
Q24.
Which of the following is true for radioisotope method?

a.

The intensity of emitted light is used to study enzyme reaction

b.

The reaction can be used to assay ATP and appropriate enzymes via coupled reaction

c.

It is restricted to separation of radiolabeled forms of substrate and product

d.

Bacterial luciferase can be assayed and measured by this method

Discuss
Answer: (c).It is restricted to separation of radiolabeled forms of substrate and product
Q25.
Which of the following is not true for radioisotope method?

a.

This method is used in studying mechanism of enzyme action and in ligand binding sites

b.

Change is absorbance is used as the basis for assaying enzyme

c.

If one product is gas, the method is easier

d.

Glutamate decarboxylase is assayed and measured using this method

Discuss
Answer: (b).Change is absorbance is used as the basis for assaying enzyme
Q26.
Which of the following enzyme is assayed and measured using radioisotope method?

a.

Hydrolases

b.

Bacterial luciferase

c.

Phosphofructokinase

d.

Glutamate decarboxylase

Discuss
Answer: (d).Glutamate decarboxylase
Q27.
___________ catalyze the reaction by accepting a proton.

a.

Acid

b.

Entrapment

c.

Enzyme

d.

Bases

Discuss
Answer: (d).Bases
Q28.
Which of the following is not true for acid base catalysis?

a.

Bases catalyze the reaction by accepting a proton

b.

Bases increases the reaction rate by increasing the nucleophilic character of the attacking group

c.

To make reactants proximal to each other

d.

Specific hydroxide ion catalysis of reaction in water is an example of this type of catalysis

Discuss
Answer: (c).To make reactants proximal to each other
Q29.
Which of the following is not true for strain distortion and conformational change?

a.

Bases increases the reaction rate by increasing the nucleophilic character of the attacking group

b.

The strain in the starting material and release of that strain provides rate acceleration in chemical reactions

c.

This experiment was carried out by Westheimer and his colleagues

d.

Rate of hydrolysis of phosphate esters in two compounds was explained by this theory

Discuss
Answer: (a).Bases increases the reaction rate by increasing the nucleophilic character of the attacking group
Q30.
Which of the following is not true for strain distortion and conformational change?

a.

Change in conformation 3D structure of a protein from low activity form to high activity form

b.

To make reactants proximal to each other

c.

Intrinsic interaction energy and geometric distortions are involved

d.

ES complex is selectively destabilized

Discuss
Answer: (b).To make reactants proximal to each other
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